Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Additional phenotypic characterization of tmem-131(tm6119) mutants.
  • Fig. S2. Fluorescent reporter screens and mutant verification for tmem-131 phenotype.
  • Fig. S3. Genetic interaction of tmem-131 with other genes involved in collagen production and degradation.
  • Fig. S4. Sequence and structural similarities of Ce-PapD-L and other known PapD domain–containing proteins.
  • Fig. S5. Verification of human TMEM131 shRNA knockdown efficiency.
  • Fig. S6. Conserved residues in TRAPPC8 and TMEM131 C termini.
  • Fig. S7. Y2H assay of interaction between PapD-L from TMEM131 and collagen family proteins with C-terminal COLFI domains.
  • Legend for table S1
  • Table S2. Reporters examined in phenotypic screens for tmem-131 RNAi.
  • Table S3. Primers and oligos used in genomic editing.
  • Table S4. List of human cDNA clones identified by Y2H screens using Ce-PapD-L as a bait.
  • Table S5. List of human cDNA clones identified by Y2H screens using TMEM131 Ct as a bait.
  • Table S6. Genes required for COL-19::GFP production in C. elegans based on RNAi.
  • Table S7. qRT-PCR primers used in this study.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of genes identified from genome-wide RNAi screen of asp-17p::GFP regulators.

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