Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Visualization of the connections between MTTs in cryo-tomograms.
  • Fig. S2. MTT to MTD transitions.
  • Fig. S3. The inner scaffold maintains centriole cohesion under compressive forces.
  • Fig. S4. Reconstruction resolutions and computational pipeline for reconstructing the complete central region.
  • Fig. S5. Assessing the correctness of the structure: Raw images versus averages.
  • Fig. S6. Periodicity of the inner scaffold stem in P. tetraurelia, C. reinhardtii, N. gruberi, and humans.
  • Fig. S7. Periodicity, helicity, and de novo subtomogram averaging.
  • Fig. S8. Optimized U-ExM protocol in human cells reveals components of the inner scaffold.
  • Fig. S9. POC1B, FAM161A, POC5, and Centrin-2 colocalize with the inner scaffold structure as simulated U-ExM fluorescence.
  • Fig. S10. 3D localization of the inner core proteins and extended localization of GFP-POC1B at the proximal region of the centriole upon overexpression.
  • Legends for movies S1 to S6
  • Legends for data files S1 and S2
  • Reference (40)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Cryo-tomogram of a P. tetraurelia centriole ex vivo.
  • Movie S2 (.avi format). Cryo-tomogram of a C. reinhardtii centriole in situ.
  • Movie S3 (.mov format). 3D representation of the P. tetraurelia central core highlighting the helical pattern of the inner scaffold.
  • Movie S4 (.mov format). 3D representation of the C. reinhardtii central core highlighting the helical pattern of the inner scaffold.
  • Movie S5 (.mov format). 3D rendering of the full central core architecture from P. tetraurelia.
  • Movie S6 (.mov format). 3D rendering of the full central core architecture from C. reinhardtii.
  • Data file S1 (.tif format). The complete central core reconstruction from P. tetraurelia.
  • Data file S2 (.tif format). The complete central core reconstruction from C. reinhardtii.

Files in this Data Supplement: