Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Nuclear translocation of LCmB is specific to blue light stimulation.
  • Fig. S2. Characterization of LINTAD-mediated gene induction in HEK 293T cells using dual-luciferase reporter system.
  • Fig. S3. LINTAD-mediated light-inducible gene expression in Jurkat cells.
  • Fig. S4. Lentiviral vectors used for T cell infection.
  • Fig. S5. Light stimulation system used for in vivo studies.
  • Fig. S6. The level of LINTAD gene activation depends on the expression level of regulators.
  • Fig. S7. Engineering of LINTAD with weak dimer helpers.
  • Fig. S8. LINTAD with weak dimer helpers in T cells.
  • Fig. S9. Design and lentiviral vectors used for in vivo cytotoxicity studies.
  • Legend for table S1
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Plasmids used in this study.
  • Movie S1 (.mp4 format). Light-induced nuclear translocation of LCB of the LINTAD system.
  • Movie S2 (.mp4 format). Light-inducible mNeonGreen expression in HEK 293T cells engineered with LINTAD gene activation system and the light-inducible mNeonGreen reporter.

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