Science Advances

Supplementary Materials

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  • Supplementary information
  • Fig. S1. The plot represents the M2SSQ transfer function fSQM2S(n¯1)=|Sinn¯1θ1|, where n¯1(ε) is given in Eq. 4.
  • Fig. S2. Dependence of ε of the sequence parameters and transfer function.
  • Fig. S3. The numerical simulation illustrates the spin dynamics during the core magnetization-to-singlet and singlet-to-magnetization blocks.
  • Fig. S4. NMR spectra and singlet filtering of model compounds at various magnetic fields.
  • Fig. S5. The influence of the offset on the NMR signal passing through the gc-M2S sequence was evaluated in experiments on AGG at 21.15 T (900 MHz).
  • Fig. S6. 1H NMR spectrum of Aβ40 in D2O at 16.48 T (~700 MHz) and 278 K using a polynomial sequence for water suppression.
  • Fig. S7. 1H NMR singlet-filtered spectra of Aβ40 in D2O at 16.48 T (~700 MHz) and 278 K using the sequence of Fig. 1 with the parameters reported in table S3 to achieve selectivity for each glycine residue.
  • Fig. S8. 1H NMR singlet-filtered spectra of Aβ40 in H2O/D2O (90%/10%) at 16.48 T (~700 MHz) and 278 K using the sequence of Fig. 1 with the parameters reported in table S3 to achieve selectivity for each glycine residue.
  • Fig. S9. TOCSY spectrum of Aβ40 in H2O/D2O (90%/10%) 16.48 T (~700 MHz) and 278 K obtained using a 60-ms DIPSI2 (ωRF = 10 kHz) mixing block.
  • Fig. S10. Singlet-filtered TOCSYs spectra of Aβ40 in H2O/D2O (90%/10%) at 16.48 T (~700 MHz) and 278 K obtained using a 45-ms DIPSI2 (ωRF = 10 kHz) mixing block.
  • Fig. S11. Tissue control experiments.
  • Table S1. The table reports the constants defining the nuclear spin Hamiltonian as well as the parameters used to set the GE-M2S sequence in the experiments on the H2N-AlanylGlycilGlycine-OH (AGG) peptide presented in figs. S4 (B to D) and S5.
  • Table S2. The table reports the constants defining the nuclear spin Hamiltonian as well as the parameters used to set the gc-M2S sequence in the experiments on 2,3-dibromotiophene presented in fig. S4 (F to H).
  • Table S3. The table reports the constants defining the nuclear spin Hamiltonian as well as the parameters used to set the gc-M2S sequence in the experiments on Aβ40 presented in Fig. 2B.
  • Table S4. The table reports parameters used to set the gc-M2S sequence in the experiments on mouse brains presented in Fig. 3.
  • Materials and methods extended
  • References (41, 42)

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