Science Advances

Supplementary Materials

The PDF file includes:

  • Table S1. Flow rate for cell loading and phase exchange.
  • Table S2. Primer sequences.
  • Table S3. Small molecules for the inhibition of molecular pathways regulating hMSC behavior; the concentrations used in this study are adapted from (59, 60).
  • Table S4. Statistical tests and P values.
  • Fig. S1. Characterization of the hMSC population.
  • Fig. S2. Formation of MBs on chip.
  • Fig. S3. Structural organization in MBs.
  • Fig. S4. Intra-MB analysis of the COX-2 signal with concentric cell layers.
  • Fig. S5. Validation of the fluorescence signal patterns.
  • Fig. S6. Hypoxia analysis within MBs.
  • Fig. S7. Intra-MB fluorescence signal distribution in individual chip.
  • Fig. S8. Ratio of Casp3+ cells per MB formed with QNZ (nMBs = 1216), DAPT (nMBs = 658), Y27 (nMBs = 709), and CytoD (nMBs = 458).

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Time lapse of MB formation.
  • Movie S2 (.avi format). Morphology of MB in 3D.
  • Movie S3 (.avi format). Representative z stack (from the bottom to the median plan of the MBs) acquired using a spinning disc confocal microcopy showing the distribution of de CD146dim (Vibrant Dil, green) and CD146bright (Vibrant DiO, red) within MBs.

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