Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. A frequency distribution of nearest-neighbor distances between 42 GldL foci found on 10 cells.
  • Fig. S2. Position of the nearest-neighbor GldL foci from the axis of rotation of eight tethered cells shown for the individual cells.
  • Fig. S3. Rotation speed of a cell plotted as a function of time.
  • Fig. S4. The rate for the “drop” in rotation speed after addition of CCCP (red linear regression fit) is four times faster than the rate of increase following removal of CCCP.
  • Fig. S5. Speed of a smooth gliding cell measured by tracking the center of mass does not change after the addition of A22.
  • Fig. S6. A cell with SprB-Cy3 and GldL-YFP labels.
  • Fig. S7. Tracking the flips displayed by gliding cells.
  • Primers used in the study
  • Computer scripts

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). A phase-contrast image stack where GldL-YFP strain displays wild-type levels of rotation. Similar to sheared and tethered wild-type cells, two cells in the field of view display rotation. Other cells attach to glass and beads coated with anti-SprB antibody. Some cells and beads display motion over short distances.
  • Movie S2 (.mov format). A tethered cell with five fluorescent GldL spots. The cell appears to rotate around a fixed axis.
  • Movie S3 (.mov format). A phase-contrast image stack showing that addition of CCCP stops rotation of a tethered cell.
  • Movie S4 (.mov format). A Cy3-tagged SprB moving along the length of a cell. For this movie, a filter that allows only Cy3 emission to pass was used.
  • Movie S5 (.mov format). An animation of the model suggesting how a molecular rack and pinion could drive gliding. The pinion is blue, the tread is green, and the adhesin SprB is magenta.
  • Movie S6 (.mov format). A cell gliding smoothly over a glass surface.
  • Movie S7 (.mov format). A gliding cell displaying flips.

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