Science Advances

Supplementary Materials

The PDF file includes:

  • Supplementary Methods
  • Supplementary Results
  • Fig. S1. Synthetic target site drive schematic diagram.
  • Fig. S2. Effects of repair fidelity on drive performance.
  • Fig. S3. Effects of Cas9 activity saturation on drive performance.
  • Fig. S4. Effects of gRNA activity variance on drive performance.
  • Fig. S5. Comparison of performance parameters for different types of homing drives with lower cleavage efficiencies.
  • Fig. S6. Genetic load.
  • Fig. S7. Number of gRNAs needed for successful population suppression with a reduced functional resistance rate.
  • Table S1. gRNA cut site sequence analysis.

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Other Supplementary Material for this manuscript includes the following:

  • Data S1 (Microsoft Excel format). Fly phenotype counts and analysis for the standard one-gRNA drive.
  • Data S2 (Microsoft Excel format). Fly phenotype counts and analysis for the one-gRNA drive with a tRNA.
  • Data S3 (Microsoft Excel format). Fly phenotype counts and analysis for the one-gRNA drive with poor right end homology.
  • Data S4 (Microsoft Excel format). Fly phenotype counts and analysis for the split drive targeting yellow.
  • Data S5 (Microsoft Excel format). Fly phenotype counts and analysis for combined drives targeting yellow.
  • Data S6 (Microsoft Excel format). Fly phenotype counts and analysis for the two-gRNA drive with close target sites.
  • Data S7 (Microsoft Excel format). Fly phenotype counts and analysis for the two-gRNA drive.
  • Data S8 (Microsoft Excel format). Fly phenotype counts and analysis for the three-gRNA drive.
  • Data S9 (Microsoft Excel format). Fly phenotype counts and analysis for the four-gRNA drive.

Files in this Data Supplement: