Science Advances

Supplementary Materials

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  • Fig. S1. A photograph to show the large-scale preparation of OXA/R837/ALG in the form of lyophilized powder.
  • Fig. S2. Photographs showing the obtained lyophilized drug composites.
  • Fig. S3. A SEM image of the gel scaffold loaded with DOX and R837.
  • Fig. S4. Photographs of anti-PDL1 loaded into the drug composite solution before and after gel formation.
  • Fig. S5. Cumulative drug release profiles of R837.
  • Fig. S6. Representative flow cytometric analysis of immunogenic death of CT26 cells and corresponding quantification results.
  • Fig. S7. Confocal immunofluorescence images of CT26 cells with OXA/ALG or OXA/R837/ALG treatment.
  • Fig. S8. Representative flow cytometric analysis of immune cell infiltration within the tumor.
  • Fig. S9. Corresponding quantification results of immune cell infiltration within the tumor based on flow cytometry data illustrated in fig. S8.
  • Fig. S10. Representative flow cytometric analysis of DC maturation in lymphonodus.
  • Fig. S11. Flow cytometry plots to show PDL1 expression on tumor cells and PD1 expression on TILs after intratumoral injection of OXA/ALG or OXA/R837/ALG.
  • Fig. S12. The corresponding quantification of PDL1 and PD1 expression levels on different types of cells within the tumors after various treatments, based on flow cytometry–measured mean fluorescence intensity.
  • Fig. S13. In vivo Cy5.5 retention.
  • Fig. S14. Time-dependent blood concentrations of OXA after injection of free OXA or OXA/ALG into CT26 tumors (means ± SD, n = 3).
  • Fig. S15. Proportions of tumor-infiltrating CD4+Foxp3+ T cells according to data in Fig. 2H.
  • Fig. S16. PDL1 expression on tumor cells in different groups of mice shown in Fig. 3.
  • Fig. S17. Cytokine levels including TNF-α, IFN-γ, IL-12p70, and IL-6 in the sera from mice measured at day 2 after different treatments shown in Fig. 3.
  • Fig. S18. T cell blocking experiments.
  • Fig. S19. Blood biochemical examination and blood routine examination of mice with OXA/R837/ALG treatment.
  • Fig. S20. Tumor slices of brains of different groups of mice after various treatments indicated in Fig. 6.

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