Science Advances

The PDF file includes:

• Fig. S1. AFM measurement of thickness and apparent stiffness of ERISM substrate and gelatin coating.
• Fig. S2. ERISM and immunostaining for actin, cortactin, and Tks5 in UM-SCC-1 cells.
• Fig. S3. Simultaneous imaging of gelatin matrix degradation and invadopodia force.
• Fig. S4. Time dependence of invadopodia force within a single cell.
• Fig. S5. Long-term measurement of invadopodia forces.
• Fig. S6. Fourier transform–based analysis of the characteristic period of force oscillation in invadopodia.
• Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.avi format). Temporal evolution of invadopodia force for a typical cell with immature invadopodia.
• Movie S2 (.avi format). Temporal evolution of invadopodia force for a typical cell with predominantly mature invadopodia.