Science Advances

Supplementary Materials

The PDF file includes:

  • Section S1. Simulation methods
  • Section S2. In vitro experiments
  • Table S1. Parameter values in real units with dimensionless values used in simulations.
  • Fig. S1. Description of agent-based model, active force versus velocity plot for a single myosin-II minifilament, and snapshots from simulation without steric interactions.
  • Fig. S2. Time series of aster density and aster strength showing fluctuations about steady state.
  • Fig. S3. Relative populations of F-actin (−)-ends near myosin-II heads help determine different aster and bundle phases.
  • Fig. S4. Density measurements of actin and myosin in our in vitro experiments.
  • Fig. S5. Characterization of the fluorophores on Abberior 775 STED nanoscope.
  • Legends for movies S1 and S2.
  • References (4850)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). A representative time course from the simulation of myosin-II minifilaments and F-actin in the stratified geometry.
  • Movie S2 (.avi format). Time-lapse images from steady-state asters in our in vitro experiments.

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