Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Platelet depletion from PBMCs.
  • Fig. S2. PBMC-driven OT1 T cell response is antigen specific and platelet dependent.
  • Fig. S3. Monocytes are key PBMC population necessary in observed platelet-dependent cross-presentation.
  • Fig. S4. Platelet-induced maturation in PBMC monocytes and cross-primed T cells.
  • Fig. S5. Platelet-matured monocytes demonstrate specific enhancement of antigen cross-presentation.
  • Fig. S6. Effect of platelet inhibitors on platelet-initiated cross-presentation.
  • Fig. S7. P- and E-selectin rescue cross-presentation in platelet-depleted PBMCs.
  • Fig. S8. rP-selectin and anti-PSGL1 mAb exhibit titratable, monocyte-specific agonist activity for initiating cross-presentation.
  • Fig. S9. Confocal microscopy of human monocytes in presence or absence of activated platelets.
  • Fig. S10. Comparison of APCs in processing apoptotic tumor cells for antigen-specific T cell proliferation.
  • Legends for movies S1 to S9

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). 3D reconstruction of murine monocyte with platelets.
  • Movie S2 (.mp4 format). 3D reconstruction of human monocyte with platelets.
  • Movie S3 (.mp4 format). Calcium flux in human monocyte upon interaction with platelets.
  • Movie S4 (.mp4 format). Calcium flux is absent in human monocyte without platelet.
  • Movie S5 (.mp4 format). Calcium flux in human monocyte upon ionomycin stimulation.
  • Movie S6 (.mp4 format). 3D construction of monocyte PSGL1 expression and distribution.
  • Movie S7 (.mp4 format). 3D reconstruction of monocyte PSGL1 around unactivated platelet.
  • Movie S8 (.mp4 format). 3D reconstruction of monocyte PSGL1 around activated platelet.
  • Movie S9 (.mov format). Cross-sections of P-selectin:PSGL1 platelet-monocyte “adhesion synapse”.

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