Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Assay schematic, coomassie gels of used proteins, tracking data and bleaching controls.
  • Fig. S2. Coomassie gels of used proteins, SEC-MALS data and minimal APC-fragment controls.
  • Fig. S3. Coomassie gel of the used protein and tracking data of immobilized motor proteins.
  • Table S1. Sequence of RNA fragments used.
  • Table S2. Labeling ratios (in percentage) of proteins and RNAs used in this study.
  • Legends for movies S1 to S9

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). The kinesin-2 KIF3A/B/KAP3 and APC transport an axonal mRNA.
  • Movie S2 (.avi format). Cotransport of APC–β2B-tubulin RNA complexes.
  • Movie S3 (.avi format). APC binds and diffuses on the microtubule lattice in the absence of kinesin-2.
  • Movie S4 (.avi format). APC–β2B-tubulin RNA complexes diffuse on the microtubule lattice.
  • Movie S5 (.avi format). APC recruits and activates the heterotrimeric kinesin-2 KIF3A/B/KAP3.
  • Movie S6 (.avi format). Single-particle tracking of transported β2B-tubulin RNA.
  • Movie S7 (.avi format). Quadruple-color movie illustrating the selectivity of the reconstituted mRNA transport system.
  • Movie S8 (.avi format). The APC-KIF3A/B/KAP3 mRNA transport system selectively transports β-actin RNA.
  • Movie S9 (.avi format). β-actin and β2B-tubulin RNAs are transported in individual packages by the APC-KIF3A/B/KAP3 complex.

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