Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. Characteristics of primer-PEG-PLA conjugates.
  • Fig. S2. TEM images of CpG MPs.
  • Fig. S3. Fluorescence confocal microscopy images of CpG MPs labeled with Cy5 via Cy5-dUTP.
  • Fig. S4. Co-stimulatory factors expression on DCs.
  • Fig. S5. Photographs and H&E staining slice images of inguinal lymph nodes.
  • Fig. S6. Immunofluorescence images of LNs (10×) collected on day 6 from C57BL/6 mice treated with PBS, soluble forms of CpG + R848 + Ag (Adpgk), CpG NP encapsulated with Ag (Adpgk), GpC NP encapsulated with R848 and Ag (Adpgk), and banNVs (CpG: 2 nmol, R848: 8 μg per mouse, Adpgk: 20 μg) on days 0, 2, and 4 (n = 3 mice per group).
  • Fig. S7. Scheme of dextramer staining and gating tree flow cytometric analysis.
  • Fig. S8. PD-1 median intensity of fluorescence gated from PD-1+ CD8+ peripheral T cells.
  • Fig. S9. Tumor challenge of C57BL6 mice after immunization of banNVs.
  • Fig. S10. Hematoxylin and eosin (H&E) staining of the spleen sections (10×) on day 6 from C57BL/6 mice (with photographs of spleens inserted) treated with PBS, soluble forms of CpG + R848 + Ag (Adpgk), CpG NP encapsulated with Ag (Adpgk), GpC NP encapsulated with R848 and Ag (Adpgk), and banNVs (CpG: 2 nmol, R848: 8 μg per mouse, Adpgk: 20 μg) on days 0, 2, and 4.
  • Fig. S11. Experimental design of immune depletion study.
  • Table S1. DNA sequences.
  • Table S2. Definition of abbreviations used in the manuscript.
  • Table S3. Tumor progression rate and regression rate.

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