Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. Chemical characterizations of the enzymatically crosslinked framework for the encapsulation of the universal RBCs.
  • Fig. S2. Stability of the engineered universal RBCs in human plasma.
  • Fig. S3. Effects of the PSA-tyramine concentration on the optimized engineering of universal RBCs to prevent antibody-mediated aggregation in multiple blood types.
  • Fig. S4. Anti-RhD antibody–mediated human RhD-positive RBC aggregation via different methods of cell surface engineering.
  • Fig. S5. Cytomechanical and biochemical analysis, Bohr coefficient of the Hb forms in different circumstances, and blood clotting function of the native and engineered RBCs.
  • Fig. S6. Organ distribution and in vivo functional profiles of the native and engineered RBCs illustrated by the imaging function of the photoacoustic system.
  • Fig. S7. Biological safety evaluation of blood transfusion with native and engineered RBCs in a mouse model.
  • Fig. S8. Rescue experiment for the hemorrhagic test via the transfusion of native and engineered RBCs in a mouse model.
  • Table S1. Routine blood assays of the mice transfused with the native and engineered RBCs.
  • Table S2. Routine blood assays for the hemorrhagic test after the transfusion with the native and engineered RBCs.

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