Science Advances

Supplementary Materials

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  • Fig. S1. WIHN wounds have higher numbers of cells with nuclear β-Cat.
  • Fig. S2. Whole wound dermis and myofibroblast comparisons in WIHN+ and WIHN wounds.
  • Fig. S3. Comparisons of macrophage/DC populations in WIHN+ and WIHN wounds.
  • Fig. S4. WD16 wound examined for macrophage marker expression.
  • Fig. S5. Lower macrophage numbers in the late wound dermis correlate with reduced wound collagen.
  • Fig. S6. SFRP4 is located within lower wound dermis ECM and macrophages.
  • Fig. S7. Hemin abrogates phagocytosis in cultured macrophages but does not alter wound healing, wound architecture, or wound macrophage numbers in vivo.
  • Fig. S8. EDA-FN, Tlr4, and integrin studies.
  • Fig. S9. Comparisons of WT, EDA-FN−/−, and EDA-FN+/− wounds.
  • Fig. S10. SFRP4 is phagocytized and degraded by CD68+ macrophages in newly wounded HS skin dermis.
  • Fig. S11. Examination of ECM components and macrophages/DCs in human normal and HS skin.
  • Fig. S12. A proposed model for macrophage phagocytosis contribution to fibrosis.
  • Table S1. Gene signatures of myofibroblast groups I to III.
  • Table S2. STRING analyses of WIHN+ and WIHN macrophage clusters.
  • Table S3. List of the top 200 genes shared by macrophage subsets from integrated macrophage/DC analysis.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Localization of external SFRP4 and SFRP4 vesicles within MerTK+ macrophage.

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