Science Advances

Supplementary Materials

The PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. Characterization and optimization of DNA patterning steps.
  • Fig. S2. Re-use of PR layer versus new PR layer for multicomponent DNA patterning.
  • Fig. S3. Tunable multiplexed surface DNA patterns.
  • Fig. S4. Stability and specificity of DNA-directed eGFP patterns.
  • Fig. S5. Optimization of PA patterning using photolithography.
  • Fig. S6. Microfabricated DNA and PA patterns support high-throughput clonal analysis of adult NSCs.
  • Fig. S7. Characterization of adult NSC viability upon labeling with lipid-oligonucleotides.
  • Fig. S8. Characterization of adult NSC fitness upon labeling with lipid-oligonucleotides.
  • Fig. S9. Spatial precision imparted by photolithography provides tight control over heterogeneous intercellular communication.
  • Fig. S10. DNA-based assembly of HUVEC patterns.
  • Fig. S11. Multicomponent DNA patterns enable controlled, high-throughput studies of adult NSC niche solid-phase ligand cues, FGF-2 and ephrin-B2, at the single-cell level.
  • Fig. S12. Soluble versus solid-phase peptide activity in adult NSCs.
  • Fig. S13. Custom cell tracking pipeline using ilastik and Fiji.
  • Fig. S14. Tracking changes in average cell occupancy within FGF-2 over time for each individual microisland.
  • Table S1. Overview of surface-patterned DNA sequences and their complementary fluorescent, cell-labeling and ligand-labeling oligonucleotides.
  • Table S2. In-depth report of experimental sample number “n”.
  • Legend for movie S1

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Example time-lapse videos of single adult NSC cultures with various spatial organizations of competing solid-phase niche ligands.

Files in this Data Supplement: