Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Scalable and cost-effective fabrication of SCQC device.
  • Fig. S2. Schematic representation of the differentiation strategy used to generate hPSC-derived CMs.
  • Fig. S3. Benchmarking of surface and intracellular marker candidates for hPSC quantification.
  • Fig. S4. Characterization and optimization of SCQC for hPSC quantification.
  • Fig. S5. Quantification of cell capture/depletion via MACS.
  • Fig. S6. Optimization of FCM and ddPCR for rare hPSC quantification.
  • Fig. S7. Isolation and characterization of live rare hPSCs.
  • Table S1. Quantification of the detection limit and linearity (R2) of FCM, SCQC, and ddPCR.
  • Table S2. General comparison of FCM, SCQC, and ddPCR for manufacturing applications.
  • Table S3. Details of antibodies and nanobeads used in this study.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Representative video clips showing the contraction of hPSC-derived CMs at day 8 using monolayer-based protocol.
  • Movie S2 (.avi format). Representative video clips showing the contraction of hPSC-derived CMs at day 12 using monolayer-based protocol.
  • Movie S3 (.avi format). Representative video clips showing the contraction of hPSC-derived CMs at day 16 using monolayer-based protocol.
  • Movie S4 (.avi format). Representative video clips showing the contraction of hPSC-derived CMs at day 10 using EB-based protocol.
  • Movie S5 (.avi format). Representative video clips showing the contraction of hPSC-derived CMs at day 20 using EB-based protocol.

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