Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Strategy of targeting the Gpr126flox allele.
  • Fig. S2. The expression of Gpr126 in different bone cells and genotyping.
  • Fig. S3. Body length and embryonic bone formation in Lysm-Cre;Gpr126fl/fl and Col2-Cre;Gpr126fl/fl was not different compared to their control littermates.
  • Fig. S4. Deletion of Gpr126 in osteoblast lineage (Osx-Cre) had little effect on osteoclastogenesis and osteoclast activity in vivo and in vitro.
  • Fig. S5. Deletion of Gpr126 in osteoblast lineage (Osx-Cre) had little effect on chondrocyte differentiation and hypertrophy.
  • Fig. S6. The expression of COLIV and Laminin-211 in osteoblast, osteoclast, and chondrocyte cells.
  • Fig. S7. Laminin-211 was not an activating ligand of Gpr126 to regulate osteoblast differentiation and mineralization under static conditions.
  • Fig. S8. The selective Wnt/β-catenin inhibitor KYA1797K had little effect on COLIV-induced osteoblast differentiation and mineralization.
  • Fig. S9. Administration of FSK had little effect on the body length, femur bone length, bone mass, and bone strength of Osx-Cre;Gpr126fl/fl mice.
  • Fig. S10. The expression of IL-6 was increased in chondrocytes treated by FSK.

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