Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Surface roughness of the Haversian bone–mimicking bioceramic scaffolds.
  • Fig. S2. Compressive modulus of the Haversian bone–mimicking bioceramic scaffolds.
  • Fig. S3. Flexural strength of the Haversian bone–mimicking bioceramic scaffolds.
  • Fig. S4. CLSM images of cocultured HBMSCs and HUVECs stained with DAPI and phalloidin.
  • Fig. S5. Cell proliferation of HBMSCs and HUVECs cultured with the extracts from the Haversian bone–mimicking bioceramic scaffolds on days 1, 3, and 7.
  • Fig. S6. The osteogenic and angiogenic gene expression of cocultured HBMSC-HUVEC seeded on scaffolds with different diameters and numbers of Haversian canals for 3 days.
  • Fig. S7. The CLSM images of rBMSCs and rSCs in monoculture group and rBMSC-rSC coculture group with the ratio of rBMSCs to rSCs being 3:7, 5:5, and 7:3 stained with DAPI (blue) and phalloidin (red).
  • Fig. S8. Cell proliferation of rBMSCs and rSCs cultured with the extracts from the Haversian bone–mimicking bioceramic scaffolds on days 1, 3, and 7.
  • Fig. S9. The neurogenic gene expression of cocultured rBMSC-rSC seeded on scaffolds with different diameters and numbers of Haversian canals for 3 days.
  • Fig. S10. New bone formation evaluated by histological analysis.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). The bottom-up printing process to fabricate Haversian bone–mimicking scaffolds for multicellular delivery.
  • Movie S2 (.avi format). The bottom-up printing process to fabricate Haversian bone–mimicking scaffolds with different numbers of Haversian canals for mechanical and porosity tests.
  • Movie S3 (.avi format). The bottom-up printing process to fabricate Haversian bone–mimicking scaffolds with different diameters of Haversian canals for mechanical and porosity tests.
  • Movie S4 (.avi format). The bottom-up printing process to fabricate Haversian bone–mimicking scaffolds with different numbers of Volkmann canals for mechanical and porosity tests.

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