Science Advances
Supplementary Materials
The PDF file includes:
- Fig. S1. SDS-PAGE of different SA variants.
- Fig. S2. SMFS measurements with direct covalent attachment of the biotinylated ddFLN4 domain to the cantilever tip.
- Fig. S3. Exemplary force extension traces.
- Fig. S4. Dynamic force spectrum.
- Fig. S5. Structure of biotin with the adjacent linker and illustration of the simulation box.
- Fig. S6. SMD force histograms.
- Fig. S7. Structure of the SA/biotin complex during L3/4 loop opening.
- Fig. S8. Angle metric for L3/4 loop opening.
- Fig. S9. Distance metric for L3/4 loop opening.
- Table S1. Fit parameters for the Bell-Evans distributions shown in the main text.
- Note S1. Fit parameters of Bell-Evans distributions.
- Note S2. Sequences of protein constructs.
Other Supplementary Material for this manuscript includes the following:
- Movie S1 (.mov format). SA’s crystal structure with highlighted amine groups.
- Movie S2 (.mov format). Exemplary SMD: Holding biotin, pulling on the C terminus of SA subunit A.
- Movie S3 (.mov format). Exemplary SMD: Holding biotin, pulling on the C terminus of SA subunit B.
- Movie S4 (.mov format). Exemplary SMD: Holding biotin, pulling on the C terminus of SA subunit C.
- Movie S5 (.mov format). Exemplary SMD: Holding biotin, pulling on the C-terminus of SA subunit D.
Files in this Data Supplement: