Science Advances

The PDF file includes:

• Fig. S1. SDS-PAGE of different SA variants.
• Fig. S2. SMFS measurements with direct covalent attachment of the biotinylated ddFLN4 domain to the cantilever tip.
• Fig. S3. Exemplary force extension traces.
• Fig. S4. Dynamic force spectrum.
• Fig. S5. Structure of biotin with the adjacent linker and illustration of the simulation box.
• Fig. S6. SMD force histograms.
• Fig. S7. Structure of the SA/biotin complex during L3/4 loop opening.
• Fig. S8. Angle metric for L3/4 loop opening.
• Fig. S9. Distance metric for L3/4 loop opening.
• Table S1. Fit parameters for the Bell-Evans distributions shown in the main text.
• Note S1. Fit parameters of Bell-Evans distributions.
• Note S2. Sequences of protein constructs.

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mov format). SA’s crystal structure with highlighted amine groups.
• Movie S2 (.mov format). Exemplary SMD: Holding biotin, pulling on the C terminus of SA subunit A.
• Movie S3 (.mov format). Exemplary SMD: Holding biotin, pulling on the C terminus of SA subunit B.
• Movie S4 (.mov format). Exemplary SMD: Holding biotin, pulling on the C terminus of SA subunit C.
• Movie S5 (.mov format). Exemplary SMD: Holding biotin, pulling on the C-terminus of SA subunit D.