PT  - JOURNAL ARTICLE
AU  - Kaskova, Zinaida M.
AU  - Dörr, Felipe A.
AU  - Petushkov, Valentin N.
AU  - Purtov, Konstantin V.
AU  - Tsarkova, Aleksandra S.
AU  - Rodionova, Natalja S.
AU  - Mineev, Konstantin S.
AU  - Guglya, Elena B.
AU  - Kotlobay, Alexey
AU  - Baleeva, Nadezhda S.
AU  - Baranov, Mikhail S.
AU  - Arseniev, Alexander S.
AU  - Gitelson, Josef I.
AU  - Lukyanov, Sergey
AU  - Suzuki, Yoshiki
AU  - Kanie, Shusei
AU  - Pinto, Ernani
AU  - Di Mascio, Paolo
AU  - Waldenmaier, Hans E.
AU  - Pereira, Tatiana A.
AU  - Carvalho, Rodrigo P.
AU  - Oliveira, Anderson G.
AU  - Oba, Yuichi
AU  - Bastos, Erick L.
AU  - Stevani, Cassius V.
AU  - Yampolsky, Ilia V.
TI  - Mechanism and color modulation of fungal bioluminescence
AID  - 10.1126/sciadv.1602847
DP  - 2017 Apr 01
TA  - Science Advances
PG  - e1602847
VI  - 3
IP  - 4
4099  - http://advances.sciencemag.org/content/3/4/e1602847.short
4100  - http://advances.sciencemag.org/content/3/4/e1602847.full
SO  - Sci Adv2017 Apr 01; 3
AB  - Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe the use of simple synthetic α-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple α-pyrones as chemiluminescent substrates.