RT Journal Article
SR Electronic
T1 Mechanism and color modulation of fungal bioluminescence
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP e1602847
DO 10.1126/sciadv.1602847
VO 3
IS 4
A1 Kaskova, Zinaida M.
A1 Dörr, Felipe A.
A1 Petushkov, Valentin N.
A1 Purtov, Konstantin V.
A1 Tsarkova, Aleksandra S.
A1 Rodionova, Natalja S.
A1 Mineev, Konstantin S.
A1 Guglya, Elena B.
A1 Kotlobay, Alexey
A1 Baleeva, Nadezhda S.
A1 Baranov, Mikhail S.
A1 Arseniev, Alexander S.
A1 Gitelson, Josef I.
A1 Lukyanov, Sergey
A1 Suzuki, Yoshiki
A1 Kanie, Shusei
A1 Pinto, Ernani
A1 Di Mascio, Paolo
A1 Waldenmaier, Hans E.
A1 Pereira, Tatiana A.
A1 Carvalho, Rodrigo P.
A1 Oliveira, Anderson G.
A1 Oba, Yuichi
A1 Bastos, Erick L.
A1 Stevani, Cassius V.
A1 Yampolsky, Ilia V.
YR 2017
UL http://advances.sciencemag.org/content/3/4/e1602847.abstract
AB Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe the use of simple synthetic α-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple α-pyrones as chemiluminescent substrates.