RT Journal Article
SR Electronic
T1 High-throughput label-free molecular fingerprinting flow cytometry
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eaau0241
DO 10.1126/sciadv.aau0241
VO 5
IS 1
A1 Hiramatsu, Kotaro
A1 Ideguchi, Takuro
A1 Yonamine, Yusuke
A1 Lee, SangWook
A1 Luo, Yizhi
A1 Hashimoto, Kazuki
A1 Ito, Takuro
A1 Hase, Misa
A1 Park, Jee-Woong
A1 Kasai, Yusuke
A1 Sakuma, Shinya
A1 Hayakawa, Takeshi
A1 Arai, Fumihito
A1 Hoshino, Yu
A1 Goda, Keisuke
YR 2019
UL http://advances.sciencemag.org/content/5/1/eaau0241.abstract
AB Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm−1) with a record-high throughput of ~2000 events/s. As a practical application of the method not feasible with conventional flow cytometry, we demonstrate high-throughput label-free single-cell analysis of the astaxanthin productivity and photosynthetic dynamics of Haematococcus lacustris.