PT  - JOURNAL ARTICLE
AU  - Iwayama, Tomoaki
AU  - Okada, Tomoko
AU  - Ueda, Tsugumi
AU  - Tomita, Kiwako
AU  - Matsumoto, Shuji
AU  - Takedachi, Masahide
AU  - Wakisaka, Satoshi
AU  - Noda, Takeshi
AU  - Ogura, Taku
AU  - Okano, Tomomichi
AU  - Fratzl, Peter
AU  - Ogura, Toshihiko
AU  - Murakami, Shinya
TI  - Osteoblastic lysosome plays a central role in mineralization
AID  - 10.1126/sciadv.aax0672
DP  - 2019 Jul 01
TA  - Science Advances
PG  - eaax0672
VI  - 5
IP  - 7
4099  - http://advances.sciencemag.org/content/5/7/eaax0672.short
4100  - http://advances.sciencemag.org/content/5/7/eaax0672.full
SO  - Sci Adv2019 Jul 01; 5
AB  - Mineralization is the most fundamental process in vertebrates. It is predominantly mediated by osteoblasts, which secrete mineral precursors, most likely through matrix vesicles (MVs). These vesicular structures are calcium and phosphate rich and contain organic material such as acidic proteins. However, it remains largely unknown how intracellular MVs are transported and secreted. Here, we use scanning electron-assisted dielectric microscopy and super-resolution microscopy for assessing live osteoblasts in mineralizing conditions at a nanolevel resolution. We found that the calcium-containing vesicles were multivesicular bodies containing MVs. They were transported via lysosome and secreted by exocytosis. Thus, we present proof that the lysosome transports amorphous calcium phosphate within mineralizing osteoblasts.