RT Journal Article
SR Electronic
T1 Osteoblastic lysosome plays a central role in mineralization
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eaax0672
DO 10.1126/sciadv.aax0672
VO 5
IS 7
A1 Iwayama, Tomoaki
A1 Okada, Tomoko
A1 Ueda, Tsugumi
A1 Tomita, Kiwako
A1 Matsumoto, Shuji
A1 Takedachi, Masahide
A1 Wakisaka, Satoshi
A1 Noda, Takeshi
A1 Ogura, Taku
A1 Okano, Tomomichi
A1 Fratzl, Peter
A1 Ogura, Toshihiko
A1 Murakami, Shinya
YR 2019
UL http://advances.sciencemag.org/content/5/7/eaax0672.abstract
AB Mineralization is the most fundamental process in vertebrates. It is predominantly mediated by osteoblasts, which secrete mineral precursors, most likely through matrix vesicles (MVs). These vesicular structures are calcium and phosphate rich and contain organic material such as acidic proteins. However, it remains largely unknown how intracellular MVs are transported and secreted. Here, we use scanning electron-assisted dielectric microscopy and super-resolution microscopy for assessing live osteoblasts in mineralizing conditions at a nanolevel resolution. We found that the calcium-containing vesicles were multivesicular bodies containing MVs. They were transported via lysosome and secreted by exocytosis. Thus, we present proof that the lysosome transports amorphous calcium phosphate within mineralizing osteoblasts.