RT Journal Article
SR Electronic
T1 Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eaaw3413
DO 10.1126/sciadv.aaw3413
VO 5
IS 12
A1 Duclos, Grant E.
A1 Teixeira, Vitor H.
A1 Autissier, Patrick
A1 Gesthalter, Yaron B.
A1 Reinders-Luinge, Marjan A.
A1 Terrano, Robert
A1 Dumas, Yves M.
A1 Liu, Gang
A1 Mazzilli, Sarah A.
A1 Brandsma, Corry-Anke
A1 van den Berge, Maarten
A1 Janes, Sam M.
A1 Timens, Wim
A1 Lenburg, Marc E.
A1 Spira, Avrum
A1 Campbell, Joshua D.
A1 Beane, Jennifer
YR 2019
UL http://advances.sciencemag.org/content/5/12/eaaw3413.abstract
AB The human bronchial epithelium is composed of multiple distinct cell types that cooperate to defend against environmental insults. While studies have shown that smoking alters bronchial epithelial function and morphology, its precise effects on specific cell types and overall tissue composition are unclear. We used single-cell RNA sequencing to profile bronchial epithelial cells from six never and six current smokers. Unsupervised analyses led to the characterization of a set of toxin metabolism genes that localized to smoker ciliated cells, tissue remodeling associated with a loss of club cells and extensive goblet cell hyperplasia, and a previously unidentified peri-goblet epithelial subpopulation in smokers who expressed a marker of bronchial premalignant lesions. Our data demonstrate that smoke exposure drives a complex landscape of cellular alterations that may prime the human bronchial epithelium for disease.