RT Journal Article
SR Electronic
T1 Aspiration-assisted bioprinting for precise positioning of biologics
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eaaw5111
DO 10.1126/sciadv.aaw5111
VO 6
IS 10
A1 Ayan, Bugra
A1 Heo, Dong Nyoung
A1 Zhang, Zhifeng
A1 Dey, Madhuri
A1 Povilianskas, Adomas
A1 Drapaca, Corina
A1 Ozbolat, Ibrahim T.
YR 2020
UL http://advances.sciencemag.org/content/6/10/eaaw5111.abstract
AB Three-dimensional (3D) bioprinting is an appealing approach for building tissues; however, bioprinting of mini-tissue blocks (i.e., spheroids) with precise control on their positioning in 3D space has been a major obstacle. Here, we unveil “aspiration-assisted bioprinting (AAB),” which enables picking and bioprinting biologics in 3D through harnessing the power of aspiration forces, and when coupled with microvalve bioprinting, it facilitated different biofabrication schemes including scaffold-based or scaffold-free bioprinting at an unprecedented placement precision, ~11% with respect to the spheroid size. We studied the underlying physical mechanism of AAB to understand interactions between aspirated viscoelastic spheroids and physical governing forces during aspiration and bioprinting. We bioprinted a wide range of biologics with dimensions in an order-of-magnitude range including tissue spheroids (80 to 600 μm), tissue strands (~800 μm), or single cells (electrocytes, ~400 μm), and as applications, we illustrated the patterning of angiogenic sprouting spheroids and self-assembly of osteogenic spheroids.