RT Journal Article
SR Electronic
T1 Surface-anchored framework for generating RhD-epitope stealth red blood cells
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eaaw9679
DO 10.1126/sciadv.aaw9679
VO 6
IS 12
A1 Zhao, Yueqi
A1 Fan, Mingjie
A1 Chen, Yanni
A1 Liu, Zhaoming
A1 Shao, Changyu
A1 Jin, Biao
A1 Wang, Xiaoyu
A1 Hui, Lanlan
A1 Wang, Shuaifei
A1 Liao, Zhaoping
A1 Ling, Daishun
A1 Tang, Ruikang
A1 Wang, Ben
YR 2020
UL http://advances.sciencemag.org/content/6/12/eaaw9679.abstract
AB Rhesus D (RhD) is one of the most important immunogenic antigens on red blood cells (RBCs). However, the supply of RhD-negative blood frequently faces critical shortages in clinical practice, and the positive-to-negative transition of the RhD antigen remains a great challenge. Here, we developed an alternative approach for sheltering the epitopes on RhD-positive RBCs using a surface-anchored framework, which is flexible but can achieve an optimal shield effect with minimal physicochemical influence on the cell. The chemical framework completely obstructed the RhD antigens on the cell surface, and the assessments of both blood transfusion in a mouse model and immunostimulation with human RhD-positive RBCs in a rabbit model confirmed the RhD-epitope stealth characteristics of the engineered RBCs. This work provides an efficient methodology for improving the cell surface for universal blood transfusion and generally indicates the potential of rationally designed cell surface engineering for transfusion and transplantation medicine.