PT  - JOURNAL ARTICLE
AU  - Li, Pengfei
AU  - Wang, Shuangchao
AU  - Zhang, Lihang
AU  - Qiu, Dewen
AU  - Zhou, Xueping
AU  - Guo, Lihua
TI  - A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions
AID  - 10.1126/sciadv.aay9634
DP  - 2020 Apr 01
TA  - Science Advances
PG  - eaay9634
VI  - 6
IP  - 14
4099  - http://advances.sciencemag.org/content/6/14/eaay9634.short
4100  - http://advances.sciencemag.org/content/6/14/eaay9634.full
SO  - Sci Adv2020 Apr 01; 6
AB  - Here, we describe a tripartite circular single-stranded (ss) DNA mycovirus, named Fusarium graminearum gemytripvirus 1 (FgGMTV1). The genome of FgGMTV1 comprises three circular ssDNA segments (DNA-A, DNA-B, and DNA-C). Sequence alignments and phylogenetic analyses showed that FgGMTV1 is nested within the family Genomoviridae. We also constructed the first infectious DNA clones of a DNA mycovirus. Our results show that DNA-A and DNA-B are mutually interdependent for their replication and are associated with severely reduced colony growth and hypovirulence. DNA-C relies on DNA-A and DNA-B for replication and is necessary for the recovery of abnormal fungal phenotypes. DNA-C also enhances the accumulation of viral DNA in infected fungi and permits stable colonization and easy transmission via conidia. This is the first multipartite DNA virus isolated from a fungus. Our phylogenetic analyses also suggest that the multipartite genome of FgGMTV1 may have evolved from a monopartite genome of an ancient genomovirus.