RT Journal Article
SR Electronic
T1 Exosome-templated nanoplasmonics for multiparametric molecular profiling
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eaba2556
DO 10.1126/sciadv.aba2556
VO 6
IS 19
A1 Wu, Xingjie
A1 Zhao, Haitao
A1 Natalia, Auginia
A1 Lim, Carine Z. J.
A1 Ho, Nicholas R. Y.
A1 Ong, Chin-Ann J.
A1 Teo, Melissa C. C.
A1 So, Jimmy B. Y.
A1 Shao, Huilin
YR 2020
UL http://advances.sciencemag.org/content/6/19/eaba2556.abstract
AB Exosomes are nanoscale vesicles distinguished by characteristic biophysical and biomolecular features; current analytical approaches, however, remain univariate. Here, we develop a dedicated platform for multiparametric exosome analysis—through simultaneous biophysical and biomolecular evaluation of the same vesicles—directly in clinical biofluids. Termed templated plasmonics for exosomes, the technology leverages in situ growth of gold nanoshells on vesicles to achieve multiselectivity. For biophysical selectivity, the nanoshell formation is templated by and tuned to distinguish exosome dimensions. For biomolecular selectivity, the nanoshell plasmonics locally quenches fluorescent probes only if they are target-bound on the same vesicle. The technology thus achieves multiplexed analysis of diverse exosomal biomarkers (e.g., proteins and microRNAs) but remains unresponsive to nonvesicle biomarkers. When implemented on a microfluidic, smartphone-based sensor, the platform is rapid, sensitive, and wash-free. It not only distinguished biomarker organizational states in native clinical samples but also showed that the exosomal subpopulation could more accurately differentiate patient prognosis.