PT  - JOURNAL ARTICLE
AU  - Jin, Huaibing
AU  - Du, Zhiqiang
AU  - Zhang, Yanjing
AU  - Antal, Judit
AU  - Xia, Zongliang
AU  - Wang, Yan
AU  - Gao, Yang
AU  - Zhao, Xiaoge
AU  - Han, Xinyun
AU  - Cheng, Yanjun
AU  - Shen, Qianhua
AU  - Zhang, Kunpu
AU  - Elder, Robert E.
AU  - Benko, Zsigmond
AU  - Fenyvuesvolgyi, Csaba
AU  - Li, Ge
AU  - Rebello, Dionne
AU  - Li, Jing
AU  - Bao, Shilai
AU  - Zhao, Richard Y.
AU  - Wang, Daowen
TI  - A distinct class of plant and animal viral proteins that disrupt mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1
AID  - 10.1126/sciadv.aba3418
DP  - 2020 May 01
TA  - Science Advances
PG  - eaba3418
VI  - 6
IP  - 20
4099  - http://advances.sciencemag.org/content/6/20/eaba3418.short
4100  - http://advances.sciencemag.org/content/6/20/eaba3418.full
SO  - Sci Adv2020 May 01; 6
AB  - Many animal viral proteins, e.g., Vpr of HIV-1, disrupt host mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1. However, it is unknown whether plant viruses may use this mechanism in their pathogenesis. Here, we report that the 17K protein, encoded by barley yellow dwarf viruses and related poleroviruses, delays G2/M transition and disrupts mitosis in both host (barley) and nonhost (fission yeast, Arabidopsis thaliana, and tobacco) cells through interrupting the function of Wee1-Cdc25-CDKA/Cdc2 via direct protein-protein interactions and alteration of CDKA/Cdc2 phosphorylation. When ectopically expressed, 17K disrupts the mitosis of cultured human cells, and HIV-1 Vpr inhibits plant cell growth. Furthermore, 17K and Vpr share similar secondary structural feature and common amino acid residues required for interacting with plant CDKA. Thus, our work reveals a distinct class of mitosis regulators that are conserved between plant and animal viruses and play active roles in viral pathogenesis.