RT Journal Article
SR Electronic
T1 A versatile depigmentation, clearing, and labeling method for exploring nervous system diversity
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eaba0365
DO 10.1126/sciadv.aba0365
VO 6
IS 22
A1 Pende, Marko
A1 Vadiwala, Karim
A1 Schmidbaur, Hannah
A1 Stockinger, Alexander W.
A1 Murawala, Prayag
A1 Saghafi, Saiedeh
A1 Dekens, Marcus P. S.
A1 Becker, Klaus
A1 Revilla-i-Domingo, Roger
A1 Papadopoulos, Sofia-Christina
A1 Zurl, Martin
A1 Pasierbek, Pawel
A1 Simakov, Oleg
A1 Tanaka, Elly M.
A1 Raible, Florian
A1 Dodt, Hans-Ulrich
YR 2020
UL http://advances.sciencemag.org/content/6/22/eaba0365.abstract
AB Tissue clearing combined with deep imaging has emerged as a powerful alternative to classical histological techniques. Whereas current techniques have been optimized for imaging selected nonpigmented organs such as the mammalian brain, natural pigmentation remains challenging for most other biological specimens of larger volume. We have developed a fast DEpigmEntation-Plus-Clearing method (DEEP-Clear) that is easily incorporated in existing workflows and combines whole system labeling with a spectrum of detection techniques, ranging from immunohistochemistry to RNA in situ hybridization, labeling of proliferative cells (EdU labeling) and visualization of transgenic markers. With light-sheet imaging of whole animals and detailed confocal studies on pigmented organs, we provide unprecedented insight into eyes, whole nervous systems, and subcellular structures in animal models ranging from worms and squids to axolotls and zebrafish. DEEP-Clear thus paves the way for the exploration of species-rich clades and developmental stages that are largely inaccessible by regular imaging approaches.