RT Journal Article
SR Electronic
T1 Molecular atlas of the adult mouse brain
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eabb3446
DO 10.1126/sciadv.abb3446
VO 6
IS 26
A1 Ortiz, Cantin
A1 Navarro, Jose Fernandez
A1 Jurek, Aleksandra
A1 Märtin, Antje
A1 Lundeberg, Joakim
A1 Meletis, Konstantinos
YR 2020
UL http://advances.sciencemag.org/content/6/26/eabb3446.abstract
AB Brain maps are essential for integrating information and interpreting the structure-function relationship of circuits and behavior. We aimed to generate a systematic classification of the adult mouse brain based purely on the unbiased identification of spatially defining features by employing whole-brain spatial transcriptomics. We found that the molecular information was sufficient to deduce the complex and detailed neuroanatomical organization of the brain. The unsupervised (non-expert, data-driven) classification revealed new area- and layer-specific subregions, for example in isocortex and hippocampus, and new subdivisions of striatum. The molecular atlas further supports the characterization of the spatial identity of neurons from their single-cell RNA profile, and provides a resource for annotating the brain using a minimal gene set—a brain palette. In summary, we have established a molecular atlas to formally define the spatial organization of brain regions, including the molecular code for mapping and targeting of discrete neuroanatomical domains.