RT Journal Article SR Electronic T1 Detection of response to tumor microenvironment–targeted cellular immunotherapy using nano-radiomics JF Science Advances JO Sci Adv FD American Association for the Advancement of Science SP eaba6156 DO 10.1126/sciadv.aba6156 VO 6 IS 28 A1 Devkota, Laxman A1 Starosolski, Zbigniew A1 Rivas, Charlotte H. A1 Stupin, Igor A1 Annapragada, Ananth A1 Ghaghada, Ketan B. A1 Parihar, Robin YR 2020 UL http://advances.sciencemag.org/content/6/28/eaba6156.abstract AB Immunotherapies, including cell-based therapies, targeting the tumor microenvironment (TME) result in variable and delayed responses. Thus, it has been difficult to gauge the efficacy of TME-directed therapies early after administration. We investigated a nano-radiomics approach (quantitative analysis of nanoparticle contrast–enhanced three-dimensional images) for detection of tumor response to cellular immunotherapy directed against myeloid-derived suppressor cells (MDSCs), a key component of TME. Animals bearing human MDSC-containing solid tumor xenografts received treatment with MDSC-targeting human natural killer (NK) cells and underwent nanoparticle contrast–enhanced computed tomography (CT) imaging. Whereas conventional CT-derived tumor metrics were unable to differentiate NK cell immunotherapy tumors from untreated tumors, nano-radiomics revealed texture-based features capable of differentiating treatment groups. Our study shows that TME-directed cellular immunotherapy causes subtle changes not effectively gauged by conventional imaging metrics but revealed by nano-radiomics. Our work provides a method for noninvasive assessment of TME-directed immunotherapy potentially applicable to numerous solid tumors.