PT  - JOURNAL ARTICLE
AU  - Luisi, Kate
AU  - Morabito, Kaitlyn M.
AU  - Burgomaster, Katherine E.
AU  - Sharma, Mayuri
AU  - Kong, Wing-Pui
AU  - Foreman, Bryant M.
AU  - Patel, Sonal
AU  - Fisher, Brian
AU  - Aleshnick, Maya A.
AU  - Laliberte, Jason
AU  - Wallace, Madison
AU  - Ruckwardt, Tracy J.
AU  - Gordon, David N.
AU  - Linton, Christine
AU  - Ruggiero, Nicole
AU  - Cohen, Jessica L.
AU  - Johnson, Russell
AU  - Aggarwal, Kunal
AU  - Ko, Sung-Youl
AU  - Yang, Eun Sung
AU  - Pelc, Rebecca S.
AU  - Dowd, Kimberly A.
AU  - O’Hagan, Derek
AU  - Ulmer, Jeffrey
AU  - Mossman, Sally
AU  - Sambor, Anna
AU  - Lepine, Edith
AU  - Mascola, John R.
AU  - Pierson, Theodore C.
AU  - Graham, Barney S.
AU  - Yu, Dong
TI  - Development of a potent Zika virus vaccine using self-amplifying messenger RNA
AID  - 10.1126/sciadv.aba5068
DP  - 2020 Aug 01
TA  - Science Advances
PG  - eaba5068
VI  - 6
IP  - 32
4099  - http://advances.sciencemag.org/content/6/32/eaba5068.short
4100  - http://advances.sciencemag.org/content/6/32/eaba5068.full
SO  - Sci Adv2020 Aug 01; 6
AB  - Zika virus (ZIKV) is the cause of a pandemic associated with microcephaly in newborns and Guillain-Barre syndrome in adults. Currently, there are no available treatments or vaccines for ZIKV, and the development of a safe and effective vaccine is a high priority for many global health organizations. We describe the development of ZIKV vaccine candidates using the self-amplifying messenger RNA (SAM) platform technology delivered by cationic nanoemulsion (CNE) that allows bedside mixing and is particularly useful for rapid responses to pandemic outbreaks. Two immunizations of either of the two lead SAM (CNE) vaccine candidates elicited potent neutralizing antibody responses to ZIKV in mice and nonhuman primates. Both SAM (CNE) vaccines protected these animals from ZIKV challenge, with one candidate providing complete protection against ZIKV infection in nonhuman primates. The data provide a preclinical proof of concept that a SAM (CNE) vaccine candidate can rapidly elicit protective immunity against ZIKV.