RT Journal Article
SR Electronic
T1 Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eaba7637
DO 10.1126/sciadv.aba7637
VO 6
IS 35
A1 Tanaka, Yoshiki
A1 Yoshikaie, Kunihito
A1 Takeuchi, Azusa
A1 Ichikawa, Muneyoshi
A1 Mori, Tomoyuki
A1 Uchino, Sayaka
A1 Sugano, Yasunori
A1 Hakoshima, Toshio
A1 Takagi, Hiroshi
A1 Nonaka, Gen
A1 Tsukazaki, Tomoya
YR 2020
UL http://advances.sciencemag.org/content/6/35/eaba7637.abstract
AB We have demonstrated that a bacterial membrane protein, YeeE, mediates thiosulfate uptake. Thiosulfate is used for cysteine synthesis in bacteria as an inorganic sulfur source in the global biological sulfur cycle. The crystal structure of YeeE at 2.5-Å resolution reveals an unprecedented hourglass-like architecture with thiosulfate in the positively charged outer concave side. YeeE is composed of loops and 13 helices including 9 transmembrane α helices, most of which show an intramolecular pseudo 222 symmetry. Four characteristic loops are buried toward the center of YeeE and form its central region surrounded by the nine helices. Additional electron density maps and successive molecular dynamics simulations imply that thiosulfate can remain temporally at several positions in the proposed pathway. We propose a plausible mechanism of thiosulfate uptake via three important conserved cysteine residues of the loops along the pathway.