PT  - JOURNAL ARTICLE
AU  - Tan, Lei
AU  - Fu, Jieni
AU  - Feng, Fan
AU  - Liu, Xiangmei
AU  - Cui, Zhenduo
AU  - Li, Bo
AU  - Han, Yong
AU  - Zheng, Yufeng
AU  - Yeung, Kelvin Wai Kwok
AU  - Li, Zhaoyang
AU  - Zhu, Shengli
AU  - Liang, Yanqin
AU  - Feng, Xiaobo
AU  - Wang, Xianbao
AU  - Wu, Shuilin
TI  - Engineered probiotics biofilm enhances osseointegration via immunoregulation and anti-infection
AID  - 10.1126/sciadv.aba5723
DP  - 2020 Nov 01
TA  - Science Advances
PG  - eaba5723
VI  - 6
IP  - 46
4099  - http://advances.sciencemag.org/content/6/46/eaba5723.short
4100  - http://advances.sciencemag.org/content/6/46/eaba5723.full
SO  - Sci Adv2020 Nov 01; 6
AB  - Preventing multidrug-resistant bacteria–related infection and simultaneously improving osseointegration are in great demand for orthopedic implants. However, current strategies are still limited to a combination of non–U.S. Food and Drug Administration–approved antibacterial and osteogenic agents. Here, we develop a food-grade probiotic–modified implant to prevent methicillin-resistant Staphylococcus aureus (MRSA) infection and accelerate bone integration. Lactobacillus casei is cultured on the surface of alkali heat–treated titanium (Ti) substrates and inactivated by ultraviolet irradiation to avoid sepsis induced by viable bacteria. This inactivated L. casei biofilm shows excellent 99.98% antibacterial effectiveness against MRSA due to the production of lactic acid and bacteriocin. In addition, the polysaccharides in the L. casei biofilm stimulate macrophages to secrete abundant osteogenic cytokines such as oncostatin M and improve osseointegration of the Ti implant. Inactivated probiotics modification can be a promising strategy to endow implants with both excellent self-antibacterial activity and osteointegration ability.