RT Journal Article
SR Electronic
T1 Engineered probiotics biofilm enhances osseointegration via immunoregulation and anti-infection
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eaba5723
DO 10.1126/sciadv.aba5723
VO 6
IS 46
A1 Tan, Lei
A1 Fu, Jieni
A1 Feng, Fan
A1 Liu, Xiangmei
A1 Cui, Zhenduo
A1 Li, Bo
A1 Han, Yong
A1 Zheng, Yufeng
A1 Yeung, Kelvin Wai Kwok
A1 Li, Zhaoyang
A1 Zhu, Shengli
A1 Liang, Yanqin
A1 Feng, Xiaobo
A1 Wang, Xianbao
A1 Wu, Shuilin
YR 2020
UL http://advances.sciencemag.org/content/6/46/eaba5723.abstract
AB Preventing multidrug-resistant bacteria–related infection and simultaneously improving osseointegration are in great demand for orthopedic implants. However, current strategies are still limited to a combination of non–U.S. Food and Drug Administration–approved antibacterial and osteogenic agents. Here, we develop a food-grade probiotic–modified implant to prevent methicillin-resistant Staphylococcus aureus (MRSA) infection and accelerate bone integration. Lactobacillus casei is cultured on the surface of alkali heat–treated titanium (Ti) substrates and inactivated by ultraviolet irradiation to avoid sepsis induced by viable bacteria. This inactivated L. casei biofilm shows excellent 99.98% antibacterial effectiveness against MRSA due to the production of lactic acid and bacteriocin. In addition, the polysaccharides in the L. casei biofilm stimulate macrophages to secrete abundant osteogenic cytokines such as oncostatin M and improve osseointegration of the Ti implant. Inactivated probiotics modification can be a promising strategy to endow implants with both excellent self-antibacterial activity and osteointegration ability.