RT Journal Article
SR Electronic
T1 Nanoscale chromatin imaging and analysis platform bridges 4D chromatin organization with molecular function
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eabe4310
DO 10.1126/sciadv.abe4310
VO 7
IS 1
A1 Li, Yue
A1 Eshein, Adam
A1 Virk, Ranya K.A.
A1 Eid, Aya
A1 Wu, Wenli
A1 Frederick, Jane
A1 VanDerway, David
A1 Gladstein, Scott
A1 Huang, Kai
A1 Shim, Anne R.
A1 Anthony, Nicholas M.
A1 Bauer, Greta M.
A1 Zhou, Xiang
A1 Agrawal, Vasundhara
A1 Pujadas, Emily M.
A1 Jain, Surbhi
A1 Esteve, George
A1 Chandler, John E.
A1 Nguyen, The-Quyen
A1 Bleher, Reiner
A1 de Pablo, Juan J.
A1 Szleifer, Igal
A1 Dravid, Vinayak P.
A1 Almassalha, Luay M.
A1 Backman, Vadim
YR 2021
UL http://advances.sciencemag.org/content/7/1/eabe4310.abstract
AB Extending across multiple length scales, dynamic chromatin structure is linked to transcription through the regulation of genome organization. However, no individual technique can fully elucidate this structure and its relation to molecular function at all length and time scales at both a single-cell level and a population level. Here, we present a multitechnique nanoscale chromatin imaging and analysis (nano-ChIA) platform that consolidates electron tomography of the primary chromatin fiber, optical super-resolution imaging of transcription processes, and label-free nano-sensing of chromatin packing and its dynamics in live cells. Using nano-ChIA, we observed that chromatin is localized into spatially separable packing domains, with an average diameter of around 200 nanometers, sub-megabase genomic size, and an internal fractal structure. The chromatin packing behavior of these domains exhibits a complex bidirectional relationship with active gene transcription. Furthermore, we found that properties of PDs are correlated among progenitor and progeny cells across cell division.