RT Journal Article
SR Electronic
T1 Intracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes
JF Science Advances
JO Sci Adv
FD American Association for the Advancement of Science
SP eabd5175
DO 10.1126/sciadv.abd5175
VO 7
IS 15
A1 Dipalo, Michele
A1 Rastogi, Sahil K.
A1 Matino, Laura
A1 Garg, Raghav
A1 Bliley, Jacqueline
A1 Iachetta, Giuseppina
A1 Melle, Giovanni
A1 Shrestha, Ramesh
A1 Shen, Sheng
A1 Santoro, Francesca
A1 Feinberg, Adam W.
A1 Barbaglia, Andrea
A1 Cohen-Karni, Tzahi
A1 De Angelis, Francesco
YR 2021
UL http://advances.sciencemag.org/content/7/15/eabd5175.abstract
AB Graphene with its unique electrical properties is a promising candidate for carbon-based biosensors such as microelectrodes and field effect transistors. Recently, graphene biosensors were successfully used for extracellular recording of action potentials in electrogenic cells; however, intracellular recordings remain beyond their current capabilities because of the lack of an efficient cell poration method. Here, we present a microelectrode platform consisting of out-of-plane grown three-dimensional fuzzy graphene (3DFG) that enables recording of intracellular cardiac action potentials with high signal-to-noise ratio. We exploit the generation of hot carriers by ultrafast pulsed laser for porating the cell membrane and creating an intimate contact between the 3DFG electrodes and the intracellular domain. This approach enables us to detect the effects of drugs on the action potential shape of human-derived cardiomyocytes. The 3DFG electrodes combined with laser poration may be used for all-carbon intracellular microelectrode arrays to allow monitoring of the cellular electrophysiological state.