Table 1 Threshold cycles for DOTS qPCR and fluorescence qPCR.

Uncertainties have been determined as the SE of repeated measurements for DOTS qPCR and as the SE of triplicate experiments for fluorescence qPCR.

N0 (genomic copies)DOTS qPCR CtFluorescence qPCR Ct
1.5 × 1053.1 ± 0.217.66 ± 0.04
1.5 × 1044.6 ± 0.321.11 ± 0.06
1.5 × 1037.5 ± 0.425.28 ± 0.07
1.5 × 1029.0 ± 0.629.88 ± 0.03
NTC14.4 ± 0.432.4 ± 0.1