Uncertainties have been determined as the SE of repeated measurements for DOTS qPCR and as the SE of triplicate experiments for fluorescence qPCR.
N0 (genomic copies) | DOTS qPCR Ct | Fluorescence qPCR Ct |
1.5 × 105 | 3.1 ± 0.2 | 17.66 ± 0.04 |
1.5 × 104 | 4.6 ± 0.3 | 21.11 ± 0.06 |
1.5 × 103 | 7.5 ± 0.4 | 25.28 ± 0.07 |
1.5 × 102 | 9.0 ± 0.6 | 29.88 ± 0.03 |
NTC | 14.4 ± 0.4 | 32.4 ± 0.1 |