Table 1 Summary of data collection and refinement statistics.

Values in parentheses are for the highest-resolution shell. PF, Photon Factory; Rmsd, root-mean-square deviation.

MaTLP-apoMaTLP-GTPMaTLP-ppptRNAPheΔ1MaTLP-ppptRNAPheΔ1-GDPNP
PDB ID5AXK5AXL5AXM5AXN
Data collection
  BeamlineSPring-8 BL41XUSPring-8 BL41XUPF BL17APF BL5A
  Space groupC2221C2221I222I222
  Unit cell parameters a, b, c (Å)98.3, 120.5, 157.4103.1, 115.7, 144.975.3, 127.6, 143.882.3, 134.1, 147.4
  Wavelength (Å)0.97801.00000.973191.0000
  Resolution range (Å)50.0–2.29 (2.43–2.29)50.0–2.99 (3.17–2.99)50.0–2.21 (2.34–2.21)50.0–2.70 (2.87–2.70)
  Rmeas (%)*8.9 (76.3)15.2 (90.0)9.7 (74.4)11.0 (87.2)
  CC1/2 (%)99.8 (80.4)99.5 (81.2)99.9 (83.6)99.9 (83.5)
  〈I/σ(I)〉14.7 (2.8)12.0 (2.6)19.4 (3.2)16.9 (2.5)
  Completeness (%)98.3 (93.8)98.8 (93.4)99.7 (98.6)99.7 (99.3)
  Redundancy6.7 (6.6)7.2 (7.2)7.4 (7.3)8.1 (8.2)
Refinement
  No. of reflections41,65017,58135,10222,669
  Rwork/Rfree (%)20.6/24.021.5/25.321.6/24.322.5/26.7
  No. of atoms
    Macromolecules3760362252475142
    Ligand/ion306836101
    Water89810216
  B-factors (Å2)
    Macromolecules57.068.445.357.3
    Ligand/ion60.586.246.659.9
    Water49.059.533.038.1
  Estimated coordinate error (Å)0.320.480.250.41
  Rmsd from ideal
    Bond lengths (Å)0.0090.0030.0030.003
    Bond angles (°)1.110.920.720.80

*Rmeas = Σhkl {N(hkl)/[N(hkl) − 1]}1/2 Σi | Ii(hkl) − 〈I(hkl)〉 |/Σhkl Σi Ii(hkl), where 〈I(hkl)〉 and N(hkl) are the mean intensity of a set of equivalent reflections and the multiplicity, respectively.

Rwork = Σhkl ||Fobs||Fcalc||hkl |Fobs|; Rfree was calculated for 5% randomly selected test sets that were not used in the refinement.