Table 3 Relative abundance of BRD-containing proteins in HEK293 cells (data taken from Proteomics DB; https://www.proteomicsdb.org/).

Pull-down of human BRD-containing proteins with biotinylated BSP (BSP-a and BSP-b), followed by competitive elution with BSP and MS, resulted in enrichment of BSP-targeted BRDs.

ProteinRelative peptide abundanceProteinRelative peptide abundance
293BSP-aBSP-b293BSP-aBSP-b
CECR2BRD74.51105
BPTF4.31BRD94.577.68
GCN5L23.75SP140L
PCAFSP140
BRD25.5982SP1003.43
BRD35.0422SP1102.71
BRD45.1732TIF1a4.91
BRDTTRIM335.34
BAZ1A5.19TRIM66
BAZ1B5.630.641.33BAZ2A4.253
BRWD33.61BAZ2B3.21
PHIP5.051.091.15MLL3.67
BRWD13.244TRIM287.05
CREBBP4.61ZMYND84.4
EP3004.87TAF116
BRD84.48TAF1L
ATAD24.650.980.87ZMYND11
ATAD2B3.680.901ASH1L
BRD13.48PB15.238.288
BRPF13.6SMARCA24.44
BRPF33.42SMARCA45.538.85