Table 1 Primers and probes for real-time PCR detection.

Nucleotide position based on the first copy of the amplicon in pMUM001 (accession numbers: IS2404, AF003002; KR, BX649209). TF, forward primer; TR, reverse primer; TP, probe.

Primer or probeSequence (5′-3′)Nucleotide positionsAmplicon size (base pairs)
IS2404 TFAAAGCACCACGCAGCATCT27746–2776259
IS2404 TRAGCGACCCCAGTGGATTG27787–27804
IS2404 TP6 FAM-CGTCCAACGCGATC-MGBNFQ27768–27781
KR TFTCACGGCCTGCGATATCA3178–319565
KR TRTTGTGTGGGCACTGAATTGAC3222–3242
KR TP6 FAM-ACCCCGAAGCACTG-MGBNFQ3199–3212