Table 1 Physicochemical characterization of PS NP.

Hydrodynamic diameter and polydispersity index (PDI) were measured by dynamic light scattering (DLS). ζ-potential was measured at pH 7.4 in 10 mM NaCl. Mucus penetration indicates the ability of the NP to penetrate CF sputum, as determined by MPT in our previous work (23, 42, 43) and fig. S3. X, no penetration; O, penetration.

Nominal size (nm)Particle typeDiameter (nm)PDIζ-Potential (mV)Mucus penetration
6060-nm PS58 ± 10.12−34 ± 2X
6060-nm PS-PEG66 ± 30.18−12 ± 1O
100100-nm PS90 ± 50.06−44 ± 3X
100100-nm PS-PEG107 ± 30.03−5 ± 1O
300300-nm PS282 ± 20.04−50 ± 2X
300300-nm PS-PEG307 ± 90.03−3 ± 1O
10001000-nm PS1025 ± 130.13−83 ± 4X
10001000-nm PS-PEG1045 ± 200.11−5 ± 1X