Table 1 Statistics of 3D reconstructions and model refinements.

RMS, root mean square.

Mature virion
(CVA10-M)
A-particle
(CVA10-A)
A-particle*
(CVA10-A*)
Procapsid
(CVA10-P)
CVA10-M-2G8CVA10-A-2G8CVA1-P-2G8
Data collection
MicroscopeTitan KriosTitan KriosTecnai F30Tecnai F30Tecnai F30Tecnai F30Tecnai F30
Voltage (kV)300300300300300300300
DetectorK2K2Falcon IIFalcon IIFalcon IIFalcon IIFalcon II
Pixel size (Å)1.361.361.1281.1281.1281.1281.128
Electron dose (Å2)60602525252525
Frames60601717171717
Defocus range
(μm)
0.5–4.70.5–4.70.6–4.30.7–4.40.4–5.90.4–5.90.5–5.7
Reconstruction
SoftwareRELION 2.0
Number of used
micrographs
17921792532543174917491809
Number of used
particles
17,09266,306941121,15836,55514,42524,764
Final resolution
(Å)
3.43.43.64.03.94.34.2
Average B factor
2)
−153.0−177.9−206.9−271.7−244.0−253.0−276.7
Model building
SoftwareCoot
Refinement
SoftwarePhenix
Model statistics
Correlation
coefficient
(around atoms)
0.84950.85440.86850.86070.86010.8685
No. of protein
atoms
630450834827808250944994
Ramachandran
outliers
0.00%0.00%0.00%0.00%0.00%0.00%
Ramachandran
favored
93.27%92.15%89.7%87.99%90.72%90.18%
Rotamer outliers0.00%0.18%0.00%0.00%0.00%0.00%
Cβ deviations000000
Clashscore2.732.494.714.842.985.78
RMS (bonds)0.00780.00520.00890.01230.00770.0089
RMS (angles)1.21.21.31.31.21.3
EMRinger score4.74.14.13.33.82.8

*A-particle from the middle band.