Table 1 Structural determination statistics.
Most ancient1R/2RExtant human
NMR distance and dihedral restraints
Distance restraints
    Total NOEs620700550
    Intraresidue222265213
    Sequential (|ij| = 1)197206168
    Medium-range (1 < |ij| < 5)14016497
    Long-range (|ij | >5)455538
    Total RDC restraints (15N-1HN)918579
Total dihedral angle restraints
    3JHNHA scalar couplings706367
    13Cα chemical shifts138142140
Structure statistics
    Average CYANA target function value (Å2)7.9 ± 0.24.2 ± 0.302.2 ± 0.2
    Violations
    Distance constraints (>0.5 Å)030
    Dihedral angle constraints (>5°)000
Deviations from idealized geometry
    Bond lengths (Å)000
    Bond angles (o)000
    Impropers (o)000
    Average pairwise RMSD (Å)*
    Backbone0.8 ± 0.20.6 ± 0.11.7 ± 0.6
    Heavy atoms1.3 ± 0.21.1 ± 0.12.2 ± 0.5
    RMSD of RDC-refined structure (Å)*
    Backbone1.3 ± 0.30.8 ± 0.21.4 ± 0.7
    Heavy atoms1.8 ± 0.21.3 ± 0.21.9 ± 0.6

*RMSD for residues 1042 to 1079 and 2065 to 2106.