Table 1 List of human cell lines used in this study.

CIMA, Centro de Investigación Médica Aplicada, Spain; CRG, Center for Genomic Regulation, Spain; EC, epithelial carcinoma; FBS, fetal bovine serum (Hyclone); IMIM, Institut Hospital del Mar d’Investigacions Mèdiques, Spain; IRB, Research Institute Barcelona, Spain; LC, leukemic cells; NaPyr, sodium pyruvate (Gibco); P/S, penicillin-streptomycin (Gibco); Qx, glutaMAX (Gibco); RPMI 1640 (Gibco); GM-CSF, granulocyte-macrophage colony-stimulating factor; h.i., heat inactivated.

Cell line nameOriginObtained fromMediaEstimated
doubling time
HELLCPuri Fortes’ lab (CIMA)90% RPMI 1640 + 10 FBS + MEM + P/S + Qx + NaPyr35 hours
NB4LCLuciano Di Croce’s lab (CRG)90% RPMI 1640 + 10 FBS + P/S + Qx35–45 hours
OCI-AML2LCPuri Fortes’ lab (CIMA)90% DMEM +10 FBS + MEM + P/S + Qx + NaPyr30–50 hours
Skno-1LCDSMZ (Deutsche Sammlung von
Mikroorganismen und Zellkulturen)
90% RPMI 1640 + 10% FBS + GM-CSF35–50 hours
K562LCLuciano Di Croce’s lab (CRG)90% RPMI 1640 + 10 FBS + P/S + Qx30–40 hours
LoVoECIMIM repository90% RPMI 1640 + 10 FBS + MEM + P/S + Qx + NaPyr48 hours
SW480ECIMIM repositoryDMEM + 10% FBS + 10 FBS + P/S + Qx25–30 hours
HT-29ECIMIM repositoryDMEM + 10% FBS + 10 FBS + P/S + Qx40–60 hours
MCF7ECCRG repositoryDMEM + 10% FBS + P/S + insulin30–72 hours
T47DECMiguel Beato’s lab (CRG)90% RPMI 1640 + 10 h.i. FBS + MEM + P/S + Qx + NaPyr30–40 hours
FaduECSalvador Aznar-Benitah’s lab (IRB)MEM + 10%FBS + P/S + PyrNa30 hours