Table 1 Plasmids used in this study.

All plasmids, as well as their complete sequences, are available through Addgene under the numbers indicated in the last column.

Plasmid namePromoter usedProteins expressedAddgene
Plasmid type 1: Origin of replication P15A, 10 to 12 copies per cell, chloramphenicol resistance
p15aC-GFPproDmyc-GFP (control)107862
p15aC-1DproDmyc-PIS107863
p15aC-4D1DDual proDmyc-PIS and myc-PI4K107864
p15aC-1D-5proDmyc-PIS-RBS-myc-PI4P5K (as operon)107865
p15aC-4D1D-5Dual proDmyc-PIS-RBS-myc-PI4P5K (as operon) and myc-PI4K107866
p15aC-3D1D-5Dual proDmyc-PIS-RBS-myc-PI4P5K (as operon)
and PI3K
121050
Plasmid type 2: Origin of replication CloDF13, 20 to 40 copies per cell, ampicillin resistance
pClodAcytCh-PIP2PHGFPDual proDmCherry and GFP-PLCδ-PH domain107867
pClodAcytCh-PIP2PHGFP-nbDual proDmCherry and GFP-PLCδ-PH domain nonbinding mutant107868
pClodAcytCh-GFP-P4MDual proDmCherry and GFP-(2×)SidM-P4M
domain
113346
pClodANL-CherryproDmCherry-nanoLuc107870
pClodANL-FGF2proDFGF2wt-nanoLuc107871
pClodANL-FGF2YEproDFGF2phosphomimic-nanoLuc107872
pClodANL-FGF2NBAAproDFGF2minimum export-nanoLuc107873
pClodANL-FGF4proDFGF4wt-nanoLuc107874
pClodANL-FGF4exporterproDFGF4exporter-nanoLuc107875
Plasmid type 3: Origin of replication pUC, ~500 copies per cell, ampicillin resistance (used during optimization)
pC51T7-proDmyc-PI4P5K-RBS-myc-PIS (as operon)107869