Table 1 Concentrations of the equilibrated constituents in the different CDN systems.

(i) CDN “X” before the addition of T1. (ii) CDN “Y” generated by the addition of T1 to CDN “X.” (iii) CDN “X” before the addition of T2. (iv) After subjecting CDN “X” to T2 and the transition to CDN “Y.” (v) CDN “Z” before the interaction with T3. (vi) After the addition of T3 and the T3-triggered substitution of CDN “Z” by CDN “Y.”

SystemConcentration (μM)
[AA′][AB′][AC′][BA′][BB′][BC′][CA′][CB′][CC′]
Substitution of CDN “X” by CDN “Y”
(i)*
(i)
0.64
(0.62)
0.41
(0.37)
0.05
(—)
0.41
(0.36)
0.57
(0.61)
0.06
(—)
0.01
(—)
0.04
(—)
0.98
(0.99)
(ii)*
(ii)
0.34
(0.31)
0.06
(—)
0.65
(0.64)
0.03
(—)
0.99
(—)§
0.01
(—)
0.6
(0.61)
0.05
(—)
0.37
(—)§
Substitution of CDN “X” by CDN “Z”
(iii)*
(iii)
0.61
(0.63)
0.38
(0.41)
0.05
(—)
0.38
(0.45)
0.58
(0.59)
0.02
(—)
0.01
(—)
0.06
(—)
1.00
(0.95)
(iv)*
(iv)
0.98
(0.97)
0.06
(—)
0.01
(—)
0.04
(—)
0.46
(0.45)
0.51
(0.47)
0.05
(—)
0.53
(0.52)
0.47
(0.52)
Substitution of CDN “Z” by CDN “Y”
(v)*
(v)
0.99
(1.04)
0.01
(—)
0.05
(—)
0.04
(—)
0.49
(0.47)
0.53
(0.56)
0.02
(—)
0.56
(0.54)
0.46
(0.51)
(vi)*
(vi)
0.32
(0.29)
0.06
(—)
0.68
(0.68)
0.03
(—)
0.98
(—)§
0.05
(—)
0.64
(0.66)
0.03
(—)
0.32
(—)§

*Data provided by the catalytic activities of the respective DNAzyme reporter units and appropriate calibration curves.

†Data provided by the electrophoretic separation of the constituent mixtures and the quantitative analysis of the separated stained bands (for details, see the Supplementary Materials).

‡No detectable bands.

§Bands cannot be evaluated due to the overlap.